Lectin Methods and Protocols provides invaluable, easily reproducible techniques suitable for application to a wide range of important biomedical problems. Taken together, the methods detailed here offer extremely useful tools for examining structural changes in glycosylation and their functional consequences for human pathophysiology Also included are step-by-step methods for the separation of human bone marrow cells for clinical use, for the assessment of the biological effects of plant lectins, and for the use of light and electron microscopy in analyzing carbohydrate structures. The techniques-fine-tuned by the researchers who developed them-apply lectins to the analysis of carbohydrates, to the isolation and quantification of glycoproteins, and to living cells. Lectin Methods and Protocols brings together a comprehensive collection of optimized methods for using lectins in biomedical research.
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